Hitrap blue manual

AS 5 Table 2. HiTrap SP HP and HiTrap Q HP columns characteristics 1 Median particle size of the cumulative volume distribution. 2 Dynamic binding capacity measured at 1 mL/min (1 min residence time) using M sodium acetate, pH 3 Dynamic binding capacity at 10% breakthrough by frontal analysis at a mobile phase velocity of cm/h in a PEEK /50 . The HiTrap ® Blue albumin isolation system can be used for the analysis of albumin-organic toxicants adducts, identification of biomarkers in serum of early rheumatoid arthritis, and has been used to deplete plasma albumin for preteomic analysis of Bothrops jararaca snake plasma. Blue Sepharose ® 6 Fast Flow or prepacked HiTrap™ Blue HP 1 mL and 5 mL columns (Figure ) can be used to remove albumin either before or after other purification steps (Table ).The albumin binds in a nonspecific manner by electrostatic and/or hydrophobic interactions with the aromatic anionic ligand, Cibacron Blue F3G-A, coupled to Sepharose ®.

HiTrap SP HP and HiTrap Q HP, 1 and 5 ml columns are listed in Table 2. Connectors supplied Usage No. supplied Union 1/16” male/ luer female For connection of syringe to HiTrap column 1 Stop plug female, 1/16” For sealing bottom of HiTrap column 2, 5 or 7. Blue Sepharose ® 6 Fast Flow or prepacked HiTrap™ Blue HP 1 mL and 5 mL columns (Figure ) can be used to remove albumin either before or after other purification steps (Table ). The albumin binds in a nonspecific manner by electrostatic and/or hydrophobic interactions with the aromatic anionic ligand, Cibacron Blue F3G-A, coupled to. HiTrap Blue HP, (5 x 1 ml) HiTrap Blue HP, (1 x 5 ml) Manual sample injection is selected in the Sample Injection page in the Method Wizard. Preparation Prepare the injection.

Manual run: start and control the system in real time instructions, refer to ÄKTA start Operating Instructions. Instruction HiTrap Blue HP. General instructions for affinity purification with HiTrap columns. Use HiTrap Blue HP 1 ml or 5 ml columns to remove host albumin from mammalian. Capto, GSTPrep, GSTrap, HiLoad, HiPrep, HiScreen, HisPrep, HisTrap, HiTrap, pump (bright green) and another for the sample pump (light blue).